PD03-04: Urinary Collagen Levels are Diagnostic for Men with Severe Lower Urinary Tract Symptoms (LUTS)
Friday, May 3, 2024 7:30 AM to 7:40 AM · 10 min. (US/Central)
303A
Abstract
Information
Full Abstract and Figures
Author Block
Richard E Caesar*, Swampscott, MA, Jill Macoska, Marissa SarnaMcCarthy, Boston, MA
Introduction
Several studies have recently shown that peri-urethral and interstitial prostatic collagen levels are elevated in men with LUTS (lower urinary tract symptoms), and that collagen deposition may contribute to urethral non-compliance, leading to LUTS. In order to translate these findings to clinical use, this study examined whether collagen protein levels are significantly elevated in the urine of men with severe LUTS.
Methods
1. Human Subjects and Specimen Procurement: IRB approval was obtained to procure urine during clinic visits from 10 men with severe LUTS (IPSS 20-35) and 10 men with mild LUTS (IPSS 0-5). All men enrolled in the study were informed of the purpose of the study and provided informed consent. Urine from men with gross hematuria, bladder stones or those who had previous prostate surgery for LUTS, were excluded from the study. 2. Sircol assays. De-identified urine samples procured at an outpatient setting and were received the same day at UMass Boston, immediately aliquoted, and placed on ice for assessment by Sircol assay, or stored for 7 days at -20 o C or -80 o C. The aliquots placed on ice were subjected to the Sircol Soluble Collagen Assay using manufacturer's protocol for cultured media (Biocolor). Stored aliquot samples were similarly processed at the conclusion of their respective storage period(s).
Results
Sircol analysis of the freshly collected (p=0.00918) and -80 o C (0.01328) showed that urinary collagen was significantly elevated in men with severe LUTS compared to controls. Freshly collected and assayed Control specimens demonstrated collage levels ranging from 0.14-5.23 ug/ml, with an average of 1.68 ug/ml (SD 1.55 ng/ml). Severe LUTS specimens demonstrated collage levels ranging from 2.06-13.47 ug/ml, with an average of 6.155.42 ug/ml (SD 4.5027 ug/ml). Analysis confined to age-matched samples from men 50-79 y/o also showed that urinary collagen was significantly elevated in men with severe LUTS compared to controls using both freshly collected (p=.01821) or -80oC frozen (p=0.02817) samples. Storage at -20 o C produced results inconsistent with those than those obtained from fresh samples, whereas storage at -80 o C produced results that were not significantly different from those obtained with fresh samples.
Conclusions
Urinary collagen is significantly elevated in men with severe LUTS and does not appear to be associated with patient age. Freshly collected urine samples or those frozen immediately at -80 o C provide the most reliable measure of urinary collagen. Though preliminary, these studies suggest that urinary collagen levels may be indicative of lower urinary tract fibrosis leading to worsening LUTS.
Source Of Funding
Funding was obtained through the Alton J. Brann Foundation Endowment
Author Block
Richard E Caesar*, Swampscott, MA, Jill Macoska, Marissa SarnaMcCarthy, Boston, MA
Introduction
Several studies have recently shown that peri-urethral and interstitial prostatic collagen levels are elevated in men with LUTS (lower urinary tract symptoms), and that collagen deposition may contribute to urethral non-compliance, leading to LUTS. In order to translate these findings to clinical use, this study examined whether collagen protein levels are significantly elevated in the urine of men with severe LUTS.
Methods
1. Human Subjects and Specimen Procurement: IRB approval was obtained to procure urine during clinic visits from 10 men with severe LUTS (IPSS 20-35) and 10 men with mild LUTS (IPSS 0-5). All men enrolled in the study were informed of the purpose of the study and provided informed consent. Urine from men with gross hematuria, bladder stones or those who had previous prostate surgery for LUTS, were excluded from the study. 2. Sircol assays. De-identified urine samples procured at an outpatient setting and were received the same day at UMass Boston, immediately aliquoted, and placed on ice for assessment by Sircol assay, or stored for 7 days at -20 o C or -80 o C. The aliquots placed on ice were subjected to the Sircol Soluble Collagen Assay using manufacturer's protocol for cultured media (Biocolor). Stored aliquot samples were similarly processed at the conclusion of their respective storage period(s).
Results
Sircol analysis of the freshly collected (p=0.00918) and -80 o C (0.01328) showed that urinary collagen was significantly elevated in men with severe LUTS compared to controls. Freshly collected and assayed Control specimens demonstrated collage levels ranging from 0.14-5.23 ug/ml, with an average of 1.68 ug/ml (SD 1.55 ng/ml). Severe LUTS specimens demonstrated collage levels ranging from 2.06-13.47 ug/ml, with an average of 6.155.42 ug/ml (SD 4.5027 ug/ml). Analysis confined to age-matched samples from men 50-79 y/o also showed that urinary collagen was significantly elevated in men with severe LUTS compared to controls using both freshly collected (p=.01821) or -80oC frozen (p=0.02817) samples. Storage at -20 o C produced results inconsistent with those than those obtained from fresh samples, whereas storage at -80 o C produced results that were not significantly different from those obtained with fresh samples.
Conclusions
Urinary collagen is significantly elevated in men with severe LUTS and does not appear to be associated with patient age. Freshly collected urine samples or those frozen immediately at -80 o C provide the most reliable measure of urinary collagen. Though preliminary, these studies suggest that urinary collagen levels may be indicative of lower urinary tract fibrosis leading to worsening LUTS.
Source Of Funding
Funding was obtained through the Alton J. Brann Foundation Endowment